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Pro-DeliverIN™ Transfection ReagentPro DeliverIN Transfection Reagent is an innovative reagent allowing intracellular delivery of biologically active proteins. This lipid based formulation is the first serum compatible reagent to deliver functional proteins into living cells. The proteins delivered inside cells retain their biological functions. Intracellular delivery of functionally active proteins Highly efficient in many primary cells and cell lines Serum compatible Biodegradable
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Pro-DeliverIN™ Transfection Reagent is an innovative reagent allowing intracellular delivery of biologically active proteins. This lipid-based formulation is the first serum compatible reagent to deliver functional proteins into living cells. The proteins delivered inside cells retain their biological functions.

 

  • Intracellular delivery of functionally active proteins

  • Highly efficient in many primary cells and cell lines

  • Serum compatible

  • Biodegradable and non-toxic

  • Easy: straightforward protocol and ready-to-use (no chemical conjugation required).

 

Pro-DeliverIN™ is provided with 100µL of R-Phycoerythrin Positive Control.

Sizes:

  • 100µL (PI10100): 50-100 assays
  • 250µL (PI10250): 125-250 assays
  • 500µL (PI10500): 250-500 assays
  • 1 mL (PI11000): 500-1000 assays
Storage: +4°C
Shipping conditions: Room temperature

 

Application

  • Suitable for primary cells and cell lines
  • Ideal for your protein delivery applications:
    • Intracellular localization studies in living cells
    • Protein-protein interactions
    • FRET studies

RECOMMENDED FOR: Intracellular delivery of proteins.

 

Results

Intracellular delivery of R-Phycoerythrin into various cell lines

Figure 1 : Intracellular delivery of R-phycoerythrin into various cell lines. 1 µg of R-Phycoerythrin was mixed with   2 µL of Pro-DeliverIN™ reagent. Complexes were then incubated with different cell lines in 24-well plates. Living cells were observed 24h later by fluorescence microscopy.

 
Pro-deliverIN Transfection reagent results

Figure 2: Pro-DeliverIN delivers fully functional proteins. HeLa cells were treated with 15 ng of active human caspase-3 and 5 µL of the Pro-DeliverIN reagent in 24-well plates. As controls, cells were treated either with 15ng of caspase 3 alone, 5 μL of Pro-DeliverIN alone or 100 nM staurosporine (positive control). After 7h of incubation, cells were stained with Annexin-FITC and propidium iodide. Apoptotic and dead cells were monitored by cytofluorimetry. 

 

GENOME EDITING

Pro-DeliverIN succeeded in transporting active CRE recombinase that induced homologous recombination consequently.

 


Homologous Recombination: HEK-293 cells were stably transfected with GFP plasmid containing LOX sequences. Active CRE recombinase Enzyme was delivered using Pro-DeliverIN reagent and GFP expression was monitored 96 H after delivery.

Pro-DeliverIN™ Transfection Reagent

Item no : 71754044841
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US$ 155.40
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