Vimentin Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9006-50, MB9006-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08670

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB: 1:2000-1:5000 ICC: 1:200
IHC: 1:200 FC: 1:50-1:100

Background: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Vimentin and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Vimentin Mouse mAb at 1:2000 dilution
Lane 1: A549 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: The embryonic stem cell lysate of Mouse, Immunohistochemical analysis of paraffin-embedded human lung tissue using vimentin antibody. Counter stained with hematoxylin., ICC staining vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Vimentin; VIM

Immunogen: This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of Vimentin.

Conjugate: Unconjugated

Modification: Unmodified