Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K062-M-48, E-BC-K062-M-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: HYP

Research Areas: Amino Acids and Proteins

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: serum, animal tissue, urine

Assay Time: 50 min

Detection Type: Colorimetric method

Sensitivity: 0.04 μg/mL

Detection Range: 0.04-10 μg/mL

Test Principle: The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.

Inter CV: 4.4

Intra CV: 1.2

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag