Osteopontin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90994-50, BS90994-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10451(Human) P10923(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: ICC IHC ELISA

All Applications: ICC:1:50-1:200
IHC:1:50-1:200
ELISA:1:5,000-1:10,000

Background: Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Osteopontin polyclonal antibody detects endogenous levels of Osteopontin protein.

Bioworld Molecular Weight: 32 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Osteopontin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SPP-1, SPP1, BNSP, OPN, PSEC0156, BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, Osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, OSTP, PSEC0156, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), Secreted phosphoprotein 1, SPP 1, SPP-1, SPP1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified