Bub3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90151-50, BS90151-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43684(Human) Q9WVA3(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IP, IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
IP:1:10-1:50
FC:1:50-1:100

Background: BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Bub3 polyclonal antibody detects endogenous levels of Bub3 protein.

Bioworld Molecular Weight: 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: HL-60
Lane 3: A431
Lane 4: Rat colon, ICC staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: BUB3, Aa2-050, AU019800, AU021329, AU043350, AW146323, BUB 3, BUB 3L, BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, bub3, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3 mitotic checkpoint protein, BUB3, BUB3L, Budding uninhibited by benomyl, Budding uninhibited by benzimidazoles 3, Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae), Budding uninhibited by benzimidazoles 3 homolog (yeast), Budding uninhibited by benzimidazoles 3 homolog, Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of, C78067, hBUB3, Mitotic checkpoint component, Mitotic checkpoint protein bub3, OTTHUMP00000020679, OTTHUMP00000020680, WD repeat type I transmembrane protein A72.5,

Immunogen: Recombinant protein corresponding to the N-terminus of human Bub3.

Conjugate: Unconjugated

Modification: Unmodified