Bmi1 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9014-50, MB9014-100

Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35226

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB: 1:1000
IHC/ICC: 1:100-1:200
FC: 1:100-1:200

Background: Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc and Pim, subsequently resulting in the progression of lymphomagenesis.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Bmi1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Bmi1 Mouse mAb at 1:1000 dilution
Lane 1: 293T whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: MCF7 whole cell lysate
Lane 5: HepG2 whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Lane 7: PC12 whole cell lysate
Lane 8: The kidney tissue lysate of Mouse
Lane 9: The kidney tissue lysate of Human
Lane 10: K562 whole cell lysate
Lane 11: The brain tissue lysate of Human, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin., ICC staining Bmi1 inA549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; BMI1; PCGF4; RNF51

Immunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of Bmi1.

Conjugate: Unconjugated

Modification: Unmodified