Recombinant MERS-CoV S-trimer Protein (R751S, C-6His)(Active)

Size: 50μg

Catalogue Number: PKSV030287-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Spike

Target Symptom: MERS-CoV S protein; MERS-CoV S-trimer Protein; MERS-CoV Spike glycoprotein; MERS-CoV S glycoprotein

Target Species: MERS

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-6His

Accession: AFS88936.1

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Activity: Immobilized MERS-CoV S-trimer Protein (R751S)-His(PKSV030287) at 5μg/ml (100 μl/well) can bind Human CD26-Fc(PKSH033696), The ED50 of Human CD26-Fc(PKSH033696) is 27.16 ng/ml.

Sequence: Tyr18-Lys1294(R751S)

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: Please contact us for more information.

Calculated MW: 145 kDa

Observed MW: 170-220 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only