RPL28 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65338-50, BS65338-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: P46779

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Background: ribosomal protein L28(RPL28) Homo sapiens Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Ribosomal Protein L28 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L28 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of various cells using Ribosomal Protein L28 Polyclonal Antibody diluted at 1：2000, Western blot analysis of lysates from A549, 293, and COLO cells, using RPL28 Antibody. The Lane on the right is blocked with the synthesized peptide., Western blot analysis of the lysates from HeLa cells using RPL28 antibody.

Alternative Name: RPL28; 60S ribosomal protein L28

Immunogen: The antiserum was produced against synthesized peptide derived from human RPL28. AA range:41-90

Conjugate: Unconjugated

Modification: Unmodified