ARF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90083-50, BS90083-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P84077(Human) P84078(Mouse) P84079(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC FC

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200
FC:1:50-1:100

Background: ADP-ribosylation factors (ARFs), are small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin, and constitute one family of the RAS superfamily. ARFs are essential and ubiquitous in eukaryotes, as they are involved in vesicular transport and functioning via phospholipase D activation. ARF proteins play a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. ARF1 is regulated by the binding and hydrolysis of GTP. Coatomer, or COPI, is a heptameric protein recruited to membranes by ARF1. Research demonstrates that guanine nucleotide exchange-activated ARF1, when located at the Golgi membrane, recruits and binds cytoplasmic COPI to the membranes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ARF1 polyclonal antibody detects endogenous levels of ARF1 protein.

Bioworld Molecular Weight: 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ARF1 on NIH/3T3 cells lysates using anti-ARF1 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.

Alternative Name: ARF1, ADP Ribosylation Factor 1, ADP-ribosylation factor 1, ARF 1, ARF1, ARF1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified