MRP-S34 (K214) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3327-50, BS3327-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P82930

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB: 1:500~1:1000
IP: 1:10~1:100

Background: Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRP-S34 (mitochondrial ribosomal protein S34), is a 218 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. The gene encoding MRP-S34 maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRP-S34 (K214) polyclonal antibody detects endogenous levels of MRP-S34 protein.

Bioworld Molecular Weight: ~ 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRP-S34 (K214) pAb at 1:1000 dilution
Lane 1: The Kidney tissue lysate of Rat (40ug)
Lane 2: The Kidney tissue lysate of Mouse (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: Panc1 whole cell lysate (40ug), Immunoprecipitation of Kidney tissue lysate of Mouse using MRP-S34 (K214) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using MRP-S34 (K214) #BS3327. “↑”（supernatant）;“↓(deposition）

Alternative Name: 28S ribosomal protein S34, mitochondrial; MRP-S34; S34mt; MRPS34

Immunogen: Synthetic peptide, corresponding to amino acids 180-226 of Human MRP-S34.

Conjugate: Unconjugated

Modification: Unmodified