CD99 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90264-50, BS90264-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14209 Human;Q8VCN6 Mouse

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: MIC2, also designated CD99, is a T cell surface protein that is involved in the aggregation of lymphocytes. Two forms of MIC2, which are differentially expressed, are produced by alternative splicing. The major form induces cellular adhesion, whereas the truncated form inhibits the adhesion process. MIC2 regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes. Overexpression of the truncated form results in downregulated expression of LFA-1. Cells with downregulated MIC2 exhibit a Hodgkin's and Reed-Sternberg (H-RS) phenotype, indicating that MIC2 plays an important role in regulating cell function and morphology.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: CD99 polyclonal antibody detects endogenous levels of CD99 protein.

Bioworld Molecular Weight: 25 kDa (predicted molecular weight: 19 kDa)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD99 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: HepG2 cell lysate, ICC staining CD99 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ER1803-81 at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Alternative Name: CD99, 12E7, Antigen identified by monoclonal 12E7, Y homolog, Antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99, CD99 antigen, CD99 molecule, CD99, Cell surface antigen 12E7, Cell surface antigen HBA 71, Cell surface antigen O13, E2 antigen, HBA71, MIC 2X, MIC 2Y, MIC2 (monoclonal antibody 12E7), MIC2, MIC2X, MIC2Y, MSK5X, Protein MIC2, Surface antigen MIC2, T cell surface glycoprotein E2, T-cell surface glycoprotein E2 antibody

Immunogen: Recombinant protein within Human CD99 aa 1-200.

Conjugate: Unconjugated

Modification: Unmodified