Glutathione Synthetase Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90587-50, BS90587-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P48637(Human) P51855(Mouse) P46413(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GSS consists of three loops projecting from an antiparallel β-sheet, a parallel β-sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that GSS may be the only member of a unique family, the crystal structure indicates that GSS belongs to the ATP-GRASP superfamily. GSS is expressed in hemocytes and nucleated cells, including the brain. GSS occurs as a homodimer. There are two steps in the production of Glutathione, begining with γ-GCS and ending with GSS. In an ATP-dependent reaction, GSS produces Glutathione from γ-glutamylcysteine and glycine precursors. Partial hepatectomy, diethyl maleate, buthionine sulfoximine, tert-butylhaydroquinone and thioacetamide increase the ex-pression of GSS, which causes an increase in Glutathione levels. An inherited autosomal recessive disorder, 5-oxoprolinuria (pyroglutamic aciduria), is caused by GSS deficiencies, which leads to central nervous system damage, hemolytic anemia, metabolic acidosis and urinary excretion of 5-oxoproline.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Glutathione Synthetase polyclonal antibody detects endogenous levels of Glutathione Synthetase protein.

Bioworld Molecular Weight: 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: Mouse colon
Lane 3: Rat liver, ICC staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GSH synthetase, GSH-S, GSS, epididymis secretory sperm binding protein Li 64p, epididymis secretory sperm binding protein Li 88n, Glutathione synthase, Glutathione synthetase, GSH S, GSH synthetase, GSH-S, GSHB, GSHS, GSS, HEL-S-64p, HEL-S-88n, MGC14098, OTTHUMP00000030711,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified