Recombinant Human PCNA protein (His tag)

Size: 100μg

Catalogue Numbers: PDEH100277-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: PCNA

Target Synonym: ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; Polymerase delta accessory protein; Proliferating cell nuclear antigen

UNIProt ID: P12004

Research Areas: Cell Biology; Epigenetics and Nuclear Signaling; Tags & Cell Markers; Cancer; Isotype/Loading Controls

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His

Accession: P12004

Background: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Sequence: Met 1-Ser 261

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 28.6 kDa

Observed MW: 38 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only