Argonaute 2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90086-50, BS90086-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UKV8(Human) Q8CJG0(Mouse) Q9QZ81(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Argonaute 2 polyclonal antibody detects endogenous levels of Argonaute 2 protein.

Bioworld Molecular Weight: 97 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7, ICC staining Argonaute 2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Argonaute2, hAgo2, eIF-2C 2, eIF2C 2, PPD, AGO2, EIF2C2, Ago 2, AGO2, Argonaute 2, argonaute 2, RISC catalytic component, Argonaute RISC catalytic component 2, Argonaute2, CTA-204B4.6, dAgo2, eIF 2C 2, eIF-2C 2, eIF2C 2, Eif2c2, Eukaryotic translation initiation factor 2C 2, Eukaryotic translation initiation factor 2C subunit 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, Protein argonaute-2, Protein slicer, Q10, Slicer protein,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified