VEGF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91433-50, BS91433-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15692(Human) Q00731(Mouse) P16612(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF IHC, IP, FC

All Applications: ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: VEGF polyclonal antibody detects endogenous levels of VEGF protein.

Bioworld Molecular Weight: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining VEGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining VEGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: VEGF-A, VPF, VEGFA, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, MGC70609, MVCD1, Vascular endothelial growth factor A, vascular endothelial growth factor A121, vascular endothelial growth factor A165, vascular endothelial growth factor, Vascular permeability factor, VEGF A, Vegf, VEGF-A, VEGF120, Vegfa, VEGFA, VPF,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified