ATPBD3 (G318) Polyclonal Antibody

Catalogue Numbers: BS2753-50, BS2753-100

Sizes: 50µl, 100µl

Alternative Name: Cytoplasmic tRNA 2-thiolation protein 1; ATphospho-binding domain-containing protein 3; Cancer-associated gene protein; Cytoplasmic tRNA adenylyltransferase 1; CTU1; ATPBD3; NCS6

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7Z7A3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Cytoplasmic tRNA 2-thiolation protein 1, plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATPBD3 (G318) polyclonal antibody detects endogenous levels of ATPBD3 protein.

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATPBD3 (G318) polyclonal antibody at 1:500 dilution
Lane 1: CT26 whole cell lysate(40ug)
Lane 2: PC12 whole cell lysate(40ug)
Lane 3: A375 whole cell lysate(40ug)
Lane 4: HEK293T whole cell lysate(40ug)
Lane 5: HCT116 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 288-248 of Human ATPBD3.

Conjugate: Unconjugated

Modification: Unmodified