USP21 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91414-50, BS91414-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UK80(Human) Q9QZL6(Mouse) B2GUX4(Rat)

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP21 (ubiquitin specific peptidase 21), also known as USP16 or USP23, is a 565 amino acid protein that belongs to the C19 peptidase family of ubiquitin carboxy-terminal hydrolases. Capable of removing ubiquitin from ubiquitinated proteins, USP21 plays a role in signal transduction and can also remove NEDD8 from NEDD8-conjugated proteins, possibly functioning to influence NEDD8-mediated protein proteolysis. Multiple isoforms of USP21 exist due to alternative splicing events.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: USP21 polyclonal antibody detects endogenous levels of USP21 protein.

Bioworld Molecular Weight: 63 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution., ICC staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: USP21, USP23, PP1490, Deubiquitinating enzyme 21, deubiquitinating enzyme 23, EC 3.1.2.15, ESTM28, MGC3394, NEDD8-specific protease, Ubiquitin carboxyl terminal hydrolase 21, ubiquitin carboxyl terminal hydrolase 23, Ubiquitin carboxyl-terminal hydrolase 21, ubiquitin specific peptidase 21, Ubiquitin specific processing protease 21, ubiquitin specific protease 16, ubiquitin specific protease 21, ubiquitin specific protease 23, Ubiquitin thioesterase 21, Ubiquitin thiolesterase 21, ubiquitin thiolesterase 23, ubiquitin-specific protease 23, formerly, Ubiquitin-specific-processing protease 21, UBP21, USP16 antibodyv Usp21, USP23, USP23, formerly, W53272,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified