Angiopoietin 2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90055-50, BS90055-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15123(Human) O35608(Mouse) O35462(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200

Background: Tie-1 and Tie-2 (also designated Tek) are novel cell surface receptor tyrosine kinases. The extracellular domain of Tie-1 has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three fibronectin type III repeats next to the transmembrane region. Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2. Preliminary biochemical analyses of Ang-1 reveal a potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is an angiogenic factor that is thought to be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression seems to be restricted to sites of vascular remodeling.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Angiopoietin 2 polyclonal antibody detects endogenous levels of Angiopoietin 2 protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Angiopoietin 2 on different cell lysate using anti-Angiopoietin 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: TF-1
Lane 2: Human liver tissue
Lane 3: Human placenta tissue, Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Angiopoietin 2 antibody. Counter stained with hematoxylin.

Alternative Name: ANG-2, ANGPT2, AGPT 2, Agpt2, ANG 2, ANG-2, ANG2, Angiopoietin 2a, Angiopoietin 2B, Angiopoietin-2, Angiopoietin2, ANGP2, ANGPT 2, Angpt2, Tie2 ligand,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified