JAB1/CSN5/COPS5 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75322-50, BS75322-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92905

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IF/ICC,1:50 - 1:100

Background: The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 37kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using JAB1/CSN5/JAB1/CSN5/COPS5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunofluorescence analysis of HeLa cells using JAB1/CSN5/JAB1/CSN5/COPS5 antibody. Blue: DAPI for nuclear staining., Immunofluorescence analysis of C6 cells using JAB1/CSN5/JAB1/CSN5/COPS5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using JAB1/CSN5/JAB1/CSN5/COPS5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: COPS5; CSN5; JAB1; MOV-34; SGN5

Immunogen: Recombinant fusion protein of human JAB1/CSN5/JAB1/CSN5/COPS5(NP_006828.2).

Conjugate: Unconjugated

Modification: Unmodified