Gemin 2 Monoclonal Antibody [2E17] is a Mouse monoclonal antibody. Gemin 2 Monoclonal Antibody [2E17] was generated using gem nuclear organelle associated protein 2 as the antigen. It reacts with Human, Mouse, and Xenopus/Amphibian. This antibody has been shown to work in applications such as: ELISA, Immunofluorescence, Flow Cytometry, Immunoprecipitation, and Western Blot.

Target Antigen

Gemin 2

Quantity

0.1ml

Clone

2E17

Host

Mouse 

Immunogen

His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).

Myeloma/fusion partners

SP2/0;

Species Reactivity

Mouse, Human, Xenopus laevis

Purification

Protein A

Format

Purified antibody (from supernatant) containing PBS 0.1% sodium azide;

Applications

Flow Cyt, ICC/IF, ELISA, WB, IP

Dilutions

Optimal antibody dilution should be determined by titration, however as a guideline try:

Flow Cyt: Use 1µg for 106 cells

WB: Use at an assay dependent dilution. Detects a band of approximately 34 kDa

References

The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Liu Q ( Cell,Sep 1997) PUBMED

Sanchez G  et al. A novel function for the survival motoneuron protein as a translational regulator. Hum Mol Genet : (2012) PUBMED 

Wu CY  et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). WB ; Mouse PUBMED

Takizawa Y et al. GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination. Nucleic Acids Res. 2010 Aug;38(15):5059-74. doi: 10.1093/nar/gkq271. Epub 2010 Apr 19. WB ; Human PMID: 20403813

Burnett BG  et al. Regulation of SMN protein stability. Mol Cell Biol 29:1107-15 (2009). WB ; Human PUBMED

Walker MP  et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Mouse PUBMED

Morency E  et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human PUBMED

Renvoisé B  et al. Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells. J Cell Sci 119:680-92 (2006) PUBMED

Research areas

Neuroscience; signal transduction;

Also known as

Component of gems 2 antibody;GEMI2_HUMAN antibody;Gemin-2 antibody;gemin2 antibody;SIP 1antibody;SIP-1 antibody;SIP1 antibody;SIP1 delta antibody;SMN interacting protein 1 antibody;SMNinteracting protein 1 delta antibody;SMN-interacting protein 1 antibody;Survival interacting protein 1antibody;Survival of motor neuron protein interacting protein 1 antibody;Survival of motor neuronprotein-interacting protein 1 antibody

Primary Description

Mouse Anti-Gemin 2 [2E17]

Catalogue Number

IQ203

Database links

Entrez Gene: 8487 Human

Entrez Gene: 66603 Mouse

Omim: 602595 Human

SwissProt: O14893 Human

SwissProt: Q9CQQ4 Mouse

SwissProt: O42260 Xenopus laevis

Unigene: 652307 Human

Unigene: 35353 Mouse

Unigene: 3305 Xenopus laevis

Specificity

Concentration

1mg/ml

Type

Isotype

IgG1

Nature

Purity