VDR Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91428-50, BS91428-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11473(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, FC

All Applications: WB:1:500-1:1,000
FC:1:50-1:100

Background: The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: VDR polyclonal antibody detects endogenous levels of VDR protein.

Bioworld Molecular Weight: 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Vitamin D Receptor on PC-3M cell and human small intestine tissue lysate using anti-Vitamin D Receptor antibody at 1/500 dilution., Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: VDR, NR1I1, 1 25 dihydroxyvitamin D3 receptor, 1, 1, 25 dihydroxyvitamin D3 receptor, 1, 25-@dihydroxyvitamin D3 receptor, 25-dihydroxyvitamin D3 receptor, Member 1, NR1I1, Nuclear receptor subfamily 1 group I member 1, PPP1R163, Protein phosphatase 1, regulatory subunit 163, VDR, VDR, Vitamin D (1, 25- dihydroxyvitamin D3) receptor, Vitamin D hormone receptor, Vitamin D nuclear receptor variant 1, Vitamin D receptor, Vitamin D3 receptor,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified