Vinculin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91441-50, BS91441-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P18206(Human) Q64727(Mouse) P85972(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP,

All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IP:1:10-1:50

Background: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Vinculin polyclonal antibody detects endogenous levels of Vinculin protein.

Bioworld Molecular Weight: 124 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Vinculin on different lysates using anti-Vinculin antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Human fetal skeletal muscle
Lane 3: Rat spleen
Lane 4: Mouse kidney, ICC staining Vinculin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MV, VCL, CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC, VINC, Vinculin,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified