NEFM Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90927-50, BS90927-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07197(Human) P08553(Mouse) P12839(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: Neurofilament-M (NF-M), for neurofilament medium polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NEFM polyclonal antibody detects endogenous levels of NEFM protein.

Bioworld Molecular Weight: 160 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution., ICC staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NF-M, NEFM, 150kDa medium, NEF3, NEFM, Neurofilament 3, NF160, NFM,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified