ABCA7 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60108-50, BS60108-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IZY2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB: 1:500~1:1000

Background: ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of widely-expressed proteins that use ATP hydrolysis to catalyze the transport of various molecules across extracellular and intracellular membranes. Eukaryotic ABC transporters are largely responsible for trafficking hydrophobic compounds either within the cell as part of a metabolic process, outside the cell for transport to other organs, or for secretion from the body. The cholesterol-responsive transporter, ABCA7, maps to human chromosome 19 and mouse chromosome 10 and has been reported as a candidate regulator of ceramide transport in epidermal lipid reorganization. High expression levels of ABCA7 have been reported in myelolymphatic tissues, reticuloendothelial cells, peripheral leukocytes, thymus, spleen and bone marrow. This expression pattern of the two alternatively-spliced isoforms also indicates an involvement in lipid homeostasis in cells of the immune system, though the complete role of ABCA7 is not yet known. Full-length type I ABCA7 has shown plasma membrane localization, while the type II splicing variant has shown expression predominantly in the endoplasmic reticulum.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ABCA7 polyclonal antibody detects endogenous levels of ABCA7 protein.

Bioworld Molecular Weight: ~ 234 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ABCA7 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of ABCA7 pAb in paraffin-embedded human lung carcinoma tissue at 1:50, showing membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ABCA7 pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ABCA7 pAb in paraffin-embedded human kidney carcinoma tissue at 1:50, showing membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: ATP-binding cassette sub-family A member 7; ABCA-SSN; Autoantigen SS-N; Macrophage ABC transporter

Immunogen: A synthetic peptide corresponding to residues in Human ABCA7.

Conjugate: Unconjugated

Modification: Unmodified