AMBP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90051-50, BS90051-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02760(Human)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The AMBP (α-1-Microglobulin/bikunin precursor) gene encodes a protein precursor, known as AMBP, that is cleaved to produce two distinct proteins, designated a-1-Microglobulin and Bikunin. α-1-Microglobulin, also known as protein HC, is a member of the lipocalin superfamily and is secreted mainly in plasma, urine and cerebrospinal fluid. Thought to have reductase/dehydrogenase activity, α-1-Microglobulin exhibits immunosuppressive properties, such as cytokine secretion and inhibition of antigen-induced lymphocyte cell proliferation, and may be involved in the reduction of biological pro-oxidants. The second protein cleavage product, designated Bikunin and also known as inter-α-trypsin inhibitor light chain, ITI-LC or urinary trypsin inhibitor, is a widely expressed protein that is stored in the granules of human connective tissue mast cells. One of many proteins in the Kunitz-type protease inhibitor family, Bikunin prevents autodigestion by exocrine enzymes, such as trypsinogen and chymo-trypsinogen, and plays a role in the antiinflammatory/antiproteinase immune response. Unlike α-1-Microglobulin, Bikunin is implicated in the pathogenesis of a number of renal diseases, such as urolithiasis.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: AMBP polyclonal antibody detects endogenous levels of AMBP protein.

Bioworld Molecular Weight: 39 kDa (Predicted band size)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of AMBP on different cell lysates using anti-AMBP antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Human liver
Lane 3: Mouse liver
Lane 4: Mouse brain, ICC staining AMBP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Protein HC, ITI-LC, AMBP, HCP, ITIL, Alpha 1 microglobulin/bikunin precursor, Alpha-1 microglycoprotein, AMBP, AMBP, Bikunin, Complex-forming glycoprotein heterogeneous in charge, EDC1, Growth inhibiting protein 19, HI 30, HI-30, HI30, IATIL, Inter alpha trypsin inhibitor light chain, ITI, ITI LC, ITI-LC, ITILC, Protein HC, Trypstatin, Uronic-acid-rich protein, UTI,

Immunogen: Recombinant protein with Human AMBP aa 120-320.

Conjugate: Unconjugated

Modification: Unmodified